Transfections allow for transient expression of a gene of interest in a target cell line and can be useful for short term studies of protein function. We specifically use this protocol with Lenti-X 293T cells, a cell line optimized for production of lentiviral vectors. Chloroquine aralen primaquine metronidazole flagyl nitrofurantoin furadantin Chloroquine phospahe absorbance nm Used as a transfection enhancement reagent, the mechanisms by which CQ enhances gene expression remain unclear. CQ is a weak base with pKas of 8.1 and 10.215 and is known to buffer the luminal pH of endosomes.16,17 The buffering activity of CQ could improve transfection efficiency by facilitating DNA release from the endocytic pathway as it may Chloroquine resistance in Plasmodium falciparum malaria results from mutations in PfCRT, a member of a unique family of transporters present in apicomplexan parasites and Dictyostelium discoideum. Mechanisms that have been proposed to explain chloroquine resistance are difficult to evaluate within malaria parasites. In gene transfection 21. TheamountofCQinpolyplexesinthe delivery of anti-miR-210 into the cancer cells, was associated with the efficient miRNA delivery as higher content of chloroquine caused increased endosomal escape 22. In another study con-ducted by Yang Hu et al. in the presence of 75 μM concentration Last Upload: June 10, 2016 Day 0: Seed Lenti-X 293T cells (this cell line is optimized for production of lentiviral vectors) Day 1 (pm): Transfect Cells Day 2 (am): 18h post transfection - Remove media, replace with fresh media Day 3 or more (am): Observe fluorescence, harvest cells, or perform your experiment *Pro-Tips* Different brands and lots of FBS can promote or inhibit transfection. This approach can be adapted for different cell lines and different transfection reagents. Chloroquine transfection mechanism Cheap Chloroquine For Sale Pay with Visa or MasterCard and others., Dictyostelium Discoideum Expresses a Malaria Chloroquine. Hydroxychloroquine and nightmaresHydroxychloroquine and amoxicillin Chloroquine has been frequently used in cell culture gene transfection studies Cotton et al. 1992, Wagner et al. 1992, Lucas et al. 1995, but has not been reported yet to be successful for enabling gene transfection in vivo. As mentioned above, the mechanisms of action of chloroquine seems to be related to elevating the intravesicular pH. Enhanced plasmid DNA transfection with lysosomotropic agents in.. Chloroquine a brand-new scenario for an old drug. Plasmodium Chloroquine Resistance and the Search for a Replacement.. Transfection is the process of introducing nucleic acids into eukaryotic cells by nonviral methods. Using various chemical or physical methods, this gene transfer technology enables the study of gene function and protein expression in a cellular environment. Chloroquine is a 4-aminoquinoline with antimalarial, anti-inflammatory, and potential chemosensitization and radiosensitization activities. Although the mechanism is not well understood, chloroquine is shown to inhibit the parasitic enzyme heme polymerase that converts the toxic heme into non-toxic hemazoin, thereby resulting in the accumulation of toxic heme within the parasite. Transfection efficiency was affected by the concentration of lysosomotropic agents. The marked transfection enhancement was observed when the amount of sucrose increased from 5 to 500 mM and the optimum transfection efficiency was found at 500 mM in all cell lines tested Fig. 2 a–c. Transgene expression was found to be very low when the cells were transfected with plasmid alone.