Chloroquine lysosome fusion

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  1. Lena2007 XenForo Moderator

    Chloroquine lysosome fusion


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    May 11, 2015 However, V-ATPase-deficient lysosomes remain competent to fuse with autophagosomes and endosomes, resulting in a time-dependent formation of giant autolysosomes. In contrast, BafilomycinA1 prevents autophagosome–lysosome fusion in these cells, and this defect is phenocopied by depletion of the Ca 2+ pump SERCA. Chloroquine alkalinizes the lysosome, thus maintaining GFP fluorescence. Leupeptin A does not affect the acidic lysosomal pH, and therefore only RFP fluorescence is observed. C Monitoring autophagosome–lysosome fusion and acidification. Chloroquine is a widely used biological research tool for studying autophagy inhibition. Research studies demon-strate that chloroquine accumulates in acidic lysosomes and increases the lysosomal pH. This inhibits lysosomal hydrolases and prevents autophagosomal fusion and degradation, which can result in apoptotic or necrotic cell death 1-4.

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    Chloroquine lysosome fusion

    New insights into autophagosome–lysosome fusion Journal., Autophagy Made Visible - Thermo Fisher Scientific

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  5. Chloroquine treatment of cells leads to accumulation of light chain 3-II LC3-II 1-3. This autophagy marker resides within autophagosomal membranes during the autophagic process and is degraded upon fusion with lysosomes. Chloroquine inhibition of these fusion events effectively blocks LC3-II degradation.

    • CST - Chloroquine.
    • Chloroquine - Cell Signaling Technology.
    • Bafilomycin - Wikipedia.

    Chloroquine is a known lysosomotropic agent that increases lysosomal pH by accumulating within lysosomes as a deprotonated weak base. To study the effects of lysosomal dysfunction in ARPE-19, it was necessary to establish an in vitro model utilizing chloroquine. Although several PIs, both in the autophagosome and lysosome, participate in the autophagosome–lysosome fusion, future studies should investigate the exact role of each PI, the timeline of PI function, and how different PIs function cooperatively during the fusion process. Chloroquine has antiviral effects, which work by increasing endosomal pH resulting in impaired virus/cell fusion that requires a low pH. 32 33 Chloroquine also seems to act as a zinc ionophore, thereby allowing extra cellular zinc to enter inside the cell and inhibit viral RNA dependant RNA polymerase.

     
  6. kunyaterix User

    This website contains 114087 drug listings as submitted to the Food and Drug Administration (FDA). Chloroquine - Uses, Dosage, Side Effects, Composition & more. DailyMed - CHLOROQUINE PHOSPHATE tablet Chloroquine phosphate C18H32ClN3O8P2 - PubChem
     
  7. serhius New Member

    Perrigo Rx Stability of alprazolam, chloroquine phosphate, cisapride, enalapril maleate, and hydralazine hydrochloride in extemporaneously compounded oral liquids. Am J Health-Syst Pharm. 1998;55181915-1920. Hydrocortisone Chong G, Decarie D, Ensom MHH. Stability of hydrocortisone in extemporaneously compounded suspensions. J Inform.

    Chloroquine Professional Patient Advice -